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According to Michael Crowley of Politico, the US has practiced at least 3 attack runs over the New Mexico desert. These runs have been flown by B-2 bombers and are meant to test the US' trump card against any attempt to procure a nuclear weapon - the Massive Ordnance Penetrator (MOP).
MOP, which is 20 feet long and weighs 15 tons, "boggles the mind" according to a former Pentagon official who spoke to Politico after watching footage of the tests.
There's no publicly available footage of these tests. But this footage of a BLU-109 in action gives an idea of how the MOP works. "Bunker buster" munitions burst through a target's defensive layering before the warhead detonates:
The BLU-109 has a 535-pound warhead and weights about a ton in total. The MOP carries around 5,300 pounds of explosives, giving it an explosive yield about an order of magnitude greater than the weapon in the video.
The MOP is the world's largest non-nuclear weapon. Designed specifically to target hardened targets, bunkers, and locations deep under ground, the MOP hits the ground at supersonic speed after being released from a B-2 bomber . Following impact, the bomb can burrow through 200 feet of earth and 60 feet of concrete before detonating.
Boeing
In the event that negotiations fail, the US is in a position to launch a series of MOP strikes against Fordow, a once-secret nuclear facility contained within a hollowed-out mountain and specially hardened against possible aerial attack. The centrifuges Fordow are capable of enriching uranium which could later be used for a nuclear weapon.
Destroying Fordow would be a difficult endeavor even despite the size and sheer force of the MOP. Politico notes that the total destruction of the facility would likely require multiple B-2s dropping MOPs at the same GPS-designated location to ensure that the bombs would be able drill through both the side of the mountain and the facility's hardened shell before detonating.
But the MOP is supposed to be used in exactly these kinds of coordinated strikes. According to The Wall Street Journal, the bomb is designed to be dropped in pairs. The first bomb is meant to clear a path for the second hit, heightening the bombs' already potent penetration capabilities.
Unnamed officials told the Journal that the MOP's devastation potential is unlike any non-nuclear weapon ever built.
The weapons have been specifically designed by the US to destroy hardened facilities within North Korea and Iran.
Should the US decide to carry out bombing runs against Iranian nuclear sites, the US could run into substantial difficulties. Russia has announced that it would be willing to sell the S-300 airIf Iran were to acquire the S-300s, Tehran would be able to set up a formidable ring of defense around its nuclear sites. This would make Iranian air defenses much more difficult to overcome, raising the scale and therefore the stakes of any US bombing run against the country's nuclear facilities.
The MOP is unique for its ability to penetrate enemy defenses, but it is not the largest bomb the US has ever built. That title goes to the T-12 Cloudmaker, a World War II-era bomb that clocked in at over 40,000 pounds.