Stephen Colbert hilariously grills Paul Ryan on whether he'll accept the GOP nomination

House Speaker Paul Ryan made his late-night debut on Tuesday to deny - again - that he's vying for the Republican nomination for president.

Ryan went on Stephen Colbert's "The Late Show" and said he does not want the Republican nomination and wouldn't accept it.

But Colbert wouldn't take no for an answer.

"So you're considering the nomination?" he asked.

After some more back-and-forth, Ryan still kept answering "no" to Colbert's questions about whether he'd consider being his party's nominee in the event of a contested convention this summer.

"It's a no," Ryan said.

Colbert then quipped: "Like a no no? Or one of those 'no-I-don't-want-to-be-speaker-of-the-House-but-I'll-accept-it-if-you-just-give-it-to-me-nos?'"

Ryan responded: "It's a no no."

Ryan has repeatedly denied interest in the Republican presidential nomination. Still, speculation surrounding his potential entry as a last-minute choice at the Republican National Convention in July reached a fever pitch over the past month, as the likelihood of a contested convention has increased.

The Republican nominee could be named at the convention if none of the three remaining candidates enter it with 1,237 delegates. Frontrunner Donald Trump needs to secure a bit more than 60% of the remaining delegates to head to the convention with 1,237.

Watch Ryan's full appearance on "The Late Show" below:

Allan Smith contributed to this report.