The makeshift medical facility, which has a capacity of 1,000 beds, was made in response to bed shortages in general hospitals across Wuhan.
A total of 1,400 doctors, nurses, and medical staff have been tasked with treating patients in the hospital. They have been sent by China's People's Liberation Army, according to the state-run Xinhua news agency.
On Tuesday, the first group of 50 patients transferred to the new hospital began treatment.
They were brought in from three different hospitals across Wuhan, including the Wuchang Hospital and Hankou Hospital.
The patients were transported by negative pressure isolation ambulances, according to the China Daily.
Negative pressure isolation systems are commonly used to transport infected patients during an epidemic.
Air ambulance isolation systems were also used during the Ebola outbreak in 2018, according to PPS.
Images, obtained by the Associated Press, show medical staff in protective suits pushing people in wheelchairs up a makeshift ramp and into the pre-fabricated structure.
The virus has killed almost 500 people and infected some 23,000 since it first emerged in December at a market in Wuhan.
The World Health Organization has declared the epidemic a global health emergency, as the first deaths outside of China were confirmed last weekend, according to The Guardian.
The second medical facility — Leishenshan Hospital — is expected to finish on Thursday.