Williams popped up during "Sorry" and danced and twerked alongside Queen Bey.
She recently spoke with the Wall Street Journal Magazine about her cameo appearance.
"I've known Bey for a long time," she said.
But even the amazing athlete was a bit tired from breaking out her moves, saying that she "got really sore from dancing."
"Lemonade" was an incredibly secretive project, and Williams kept her cameo hidden for months.
"If you tell me, 'Don't say anything,' I won't say anything," Williams told the Magazine.
Her friend and fellow competitor Caroline Wozniacki didn't even know Williams was involved until she saw it.
The video for "Sorry" was released Wednesday on YouTube. Check it out below: