At the interbank foreign exchange, the domestic unit opened at 82.32 against the dollar, then lost further ground to 82.35, registering a loss of 14 paise over its previous close.
In a highly volatile session, the local unit also touched 82.28 against the American currency in initial deals.
On Tuesday, the rupee rebounded from its all-time low to close 19 paise higher at 82.21 against the US dollar.
"Most Asian and emerging market peers were weaker this Wednesday and will weigh on sentiments," Sriram Iyer, Senior Research Analyst at Reliance Securities said, adding that investors will remain cautious ahead of the release of key domestic macro data.
India's inflation, IIP and manufacturing output are due today, while the more important FOMC minutes are scheduled for late evening.
"Investors will also await a key US inflation report tomorrow that is expected to influence the Federal Reserve's monetary policy stance," Iyer said, adding that the RBI will remain proactive if the rupee's volatility increases during the day.
Meanwhile, the
Brent crude futures, the global oil benchmark, fell 0.67 per cent to USD 93.66 per barrel.
In the domestic equity market, the 30-share BSE Sensex was trading 229.6 points or 0.40 per cent up at 57,376.92, and the broader NSE Nifty rose 71.60 points or 0.42 per cent to 17,055.15.
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