This remark has now cost him support from some key advocacy groups as well as a platform to speak at some events.
The London mayor's comments - which he made in an opinion piece for The Sun over the weekend in response to the President's London visit - have cost "Vote Leave" the support of the Africans for Britain organisation, which said it could no longer endorse a Brexit in light of Johnson's remarks.
The African advocacy group had previously backed Britain leaving the 28-nation bloc as it said doing so would encourage more trade with African and Caribbean nations. However, the group announced on Twitter on Saturday that it would no longer campaign for a Brexit because of Johnson's "alarming" remarks, and said it would be adopting a neutral position.
5) We backed #Brexit and @vote_leave which we formally endorsed, with the belief that the campaign would be fought with a positive message
- AfricansForBritain (@Africa4Britain) April 23, 2016
12) The comments issued by @MayorofLondon and endorsed by @Nigel_Farage, the biggest figures of #Brexit are very alarming to us
- AfricansForBritain (@Africa4Britain) April 23, 2016
As of today, we are adopting a neutral position on #Euref wishing both sides all the best and to conduct a dignified campaign for #Britain
- AfricansForBritain (@Africa4Britain) April 23, 2016
Next part of #borisjohnson backlash of his Obama ancestry comments. Kings College letter withdrawing #EUref invite pic.twitter.com/OUYyQyYDw1
- Ryan Heath (@PoliticoRyan) April 24, 2016
Dear Mr Johnson,
Given your inappropriate comments and inferences towards President Obama's Kenyan heritage, of which he is rightly proud, and your general tone of disrespect over the past few days in relation to the President of the United States of America, we are now formally withdrawing your invitation at Kings College London.
We are looking forward to providing a forum for both sides in the EU Referendum Debate to argue their point of view without fear or favour. The level of discourse over the past few days does not meet the bar we set for these events nor do we feel does it help the British people in making the most momentous decision of our lifetime. Furthermore we believe it does not reflect the true greatness of the United Kingdom, a land of tolerance, respect and fair play towards all.