1. Mario - "Donkey Kong" (1981)
2. Donkey Kong - "Donkey Kong" (1981)
3. Link - "The Legend of Zelda" (1986)
4. Samus - "Metroid" (1986)
5. Yoshi - "Super Mario World" (1990)
6. Kirby - "Kirby's Dream Land" (1992)
7. Fox McCloud - "Star Fox" (1993)
8. Pikachu - "Pokémon Red and Blue" (1996)
9. Luigi - "Mario Bros." (1983)
10. Ness - "Earthbound" (1994)
11. Captain Falcon - "F-Zero" (1990)
12. Jigglypuff - "Pokémon Red and Blue" (1996)
13. Peach - "Super Mario Bros." (1985)
14. Bowser - "Mario Bros." (1983)
15. Ice Climbers - "Ice Climber" (1985)
16. Sheik - "The Legend of Zelda: Ocarina of Time" (1998)
17. Princess Zelda - "The Legend of Zelda" (1986)
18. Dr. Mario - "Dr. Mario" (1990)
19. Pichu - "Pokémon Gold and Silver"
20. Falco Lombardi - "Star Fox" (1993)
21. Marth - "Fire Emblem" (1992)
22. Young Link - "The Legend of Zelda" (1986)
23. Ganondorf - "The Legend of Zelda" (1986)
24. Mewtwo - "Pokémon: Red & Blue" (1996)
25. Roy - "Fire Emblem: The Binding Blade" (2002)
26. Mr Game & Watch - "Ball" (1980)
27. Metaknight - "Kirby's Adventure" (1993)
28. Pit - "Kid Icarus" (1986)
29. Zero Suit Samus - "Metroid: Zero Mission" (2004)
30. Wario - "Super Mario Land 2" (1992)
31. Solid Snake - "Metal Gear" (1987)
32. Ike - "Fire Emblem: Path of Radiance" (2005)
33, 34, 35. Pokemon Trainer with Ivysaur, Squirtle, and Charizard - "Pokémon: Red & Blue" (1996)
36. Diddy Kong - "Donkey Kong Country" (1994)
37. Lucas - "Mother 3" (2006)
38. Sonic - "Sonic the Hedgehog" (1991)
39. King Dedede - "Kirby's Dream Land" (1992)
40. Olimar - "Pikmin" (2001)
41. Lucario - "Pokémon: Diamond and Pearl" (2006)
42. R.O.B (Robotic Operating Buddy) - "Stack-Up" (1985)
43. Toon Link - "The Legend of Zelda: The Wind Waker" (2002)
44. Wolf O'Donnell - "Star Fox 64" (1997)
45. Villager - "Animal Crossing" (2001)
46. Mega Man - "Mega Man" (1987)
47. Wii Fit Trainer - "Wii Fit" (2007)
48. Rosalina & Luma - "Super Mario Galaxy" (2007)
49. Little Mac - "Mike Tyson's PunchOut!!" (1987)
50. Greninja - "Pokémon X and Y" (2013)
51, 52, 53. Mii Gunner, Mii Fighter, Mii Swordsman - "Super Smash Bros. for Wii U/3DS" (2014)
54. Palutena - "Kid Icarus" (1986)
55. Pac-Man - "Pac-Man" (1980)
56. Robin - "Fire Emblem: Awakening" (2012)
57. Shulk - "Xenoblade Chronicles" (2010)
58. Bowser Jr. - "Super Mario Sunshine" (2002)
59. Duck Hunt - "Duck Hunt" (1984)
60. Lucina - "Fire Emblem Awakening" (2012)
61. Dark Pit - "Kid Icarus Uprising" (2012)
62. Ryu - "Street Fighter" (1987)
63. Cloud Strife - "Final Fantasy VII" (1997)
64. Corrin - Fire Emblem Fates" (2015)
65. Bayonetta - "Bayonetta" (2009)
66. Inkling - "Splatoon" (2015)
67. Princess Daisy - "Super Mario Land" (1989)
68. Dark Samus - "Metroid Prime 2: Echoes" (2004)
69. Chrom - "Fire Emblem Awakening" (2012)
70. Ridley - "Metroid" (1986)
71. Simon Belmont - "Castlevania II: Simon's Quest" (1987)
72. Simon Belmont - "Rondo of Blood" (1993)
73. King K. Rool - "Donkey Kong Country" (1994)
74. Isabelle - "Animal Crossing: New Leaf" (2012)
75. Ken - "Street Fighter" (1987)
76. Incineroar (Pokémon: Sun and Moon)
77. Piranha Plant - "Super Mario Bros." (1985)
The Piranha Plant won't be available when the game releases on December 7th, but Nintendo will make it available as a free download for anybody who buys the game and registers it to their My Nintendo account before the end of January.
