In Jerusalem itself, Palestinian protestors gathered at the city's ancient Damascus Gate where they were met by Israeli police. The Palestinian Authority called for a general strike on Thursday, and Palestinians complied — shops in the Old City remain closed.
Outside of Jerusalem, thousands of people took to the streets in the West Bank, with the largest demonstrations taking place in Ramallah and Bethlehem.
Protestors marching near Ramallah were met with tear gas and rubber bullets fired by Israeli soldiers. In response, Palestinians threw rocks and burned tires.
In one alleged incident, a car with Israeli plates plowed into a crowd of Palestinian protestors in the West Bank town of Beit Jala. Nine people were injured as a result.
next slide will load in 15 secondsSkip AdSkip AdIn the Gaza Strip, Hamas called on Palestinians to initiate a third intifada, or uprising. Two rockets were reportedly fired at Israel from the strip, but did not reach their target.
At least 31 people were injured in Thursday's clashes, with one critical injury. Four of these injuries occurred when Israeli soldiers opened fire with live ammunition in Khan Younis in southern Gaza.
The demonstrations also extended beyond Israel and the Palestinian territories — in Cairo, protestors directed their rage at not just Trump, but also Egyptian President Abdel Fattah al-Sisi and Israeli Prime Minister Benjamin Netanyahu.
Protestors in Ankara, Turkey demonstrated in front of the US embassy where American marines remained on high alert.
Demonstrators also aired their grievances in Amman, Jordan, which sits not far from Israel and the West Bank.
next slide will load in 15 secondsSkip AdSkip AdAnti-American sentiment was high in Tunis, Tunisia. Protestors burned US flags and called for Palestinian control of Jerusalem.
Trump's announcement stoked tensions as far away from Jerusalem as Pakistan. Supporters of an Islamist party in Karachi burned American and Israeli flags in protest.
Demonstrators also burned US flags in Peshawar, Pakistan, which has close connections with neighboring Afghanistan.
Sunnis also gathered in Baghdad, Iraq to protest Trump's move. "Jerusalem is our identity, Jerusalem is Arab," one of the protest leaders said.