The Hinduja family have maintained their position at the top of the list for the fifth year in a row.
Gopichand and Srichand Hinduja are now worth £22 billion, up from £19 billion last year.
They are two of four brothers who control the Hinduja Group, a multinational empire with businesses ranging from trucks to banking and gulf oil.
The group was started by Parmanand Deepchand Hinduja. Seven members of the third generation now work in the group, which is spread over five continents.
Srichand, born in 1935, Gopi, born in 1940, and Prakash Parmanand, born in 1945, live in London. Their youngest brother, Ashok, lives in Mumbai.
The family also added a further £3 billion to their total net worth in the past year, taking them to an astounding total net worth of £22 billion.
Of the family-run business, GP Hinduja, co-chair of the group, said: "The third generation has turned out more intelligent, smarter and more capable than any of us."