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He was obviously emotional, but he was also thankful and respectful in discussing his career accomplishments, those who helped him, and his future after football.
While it was generally a heavy-hearted affair, Peyton did have time to make a quick joke about his most dubious record in the NFL - his rookie record for interceptions in a season.
As a rookie in 1998, Manning threw 28 interceptions, a record that still stands today.
"I set the NFL rookie record [for] interceptions - a record that I still hold today," Manning began.
"Every year, I pull for a rookie quarterback to break that record. Andrew Luck, Matthew Stafford, Eli Manning, Cam Newton. I still kid Eli that he would've broken it if he had started all 16 games."
The jab earned a chuckle from those in attendance, but he may not have been totally joking. In 2014, he made a similar comment about hoping another rookie quarterback throws at least 29 interceptions to take his name off the record books.
Watch the bit below: