AP Photo/Pablo Martinez Monsivais
"I've got three certainties in life: Death, taxes, and attacks from Breitbart," Ryan said during an interview hosted by the Associated Press. "That's just something I've learned to live with in my life."
The speaker dodged congressional correspondent Erica Werner's question about whether the right-wing news outlet is a "positive or negative force."
"I'm not going to mess with that," Ryan said. "I just don't pay attention to this stuff. It really just doesn't get to me anymore."
He added: "I don't even pay attention to what they do. So to be honest with you, Erica, I don't even know what they do, so I can't answer your question because it doesn't matter to me."
Breitbart has long published stories casting Ryan in a negative light.
In March, the website published private audio of a call from last year in which Ryan said he was "not going to defend Donald Trump."
An August story from Matthew Boyle said Ryan had "thrown in with leftists to rip President Donald J. Trump for pardoning Sheriff Joe Arpaio," while newly reinstated Breitbart chief Steve Bannon said in an interview that Ryan and Senate Majority Leader Mitch McConnell were "trying to nullify the 2016 election."